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Eukaryotic Gene Expression - basics and benefits

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  • Course Description

    The objective of this course is to expose the viewer to basic aspects of regulation of gene expression in eukaryotes and explain the benefits that have accrued out of basic research in this area. The course is organized such that the viewer will learn not only gain knowledge on the molecular mechanisms involved in eukaryotic gene regulation but also will appreciate the benefits that have accrued through the exploitation of knowledge gained from the basic research, primarily in the areas of disease diagnosis and their prevention, agriculture etc. Through this course the instructor wishes to convey the message that knowledge of eukaryotic gene regulation is essential for understanding and appreciating a number of biotechnological applications in the areas of medicine and agriculture.

    About Prof. P.N. Rangarajan

    Note: Contents for this page are Licensed from http://nptel.iitm.ac.in/ under the Creative Commons Attribution Share-Alike license.

    School
    Indian Institute of Science

    Date Taught
    -0001

  • Module No.

    Topic/s

    Lectures

    1

    CIS-ACTING ELEMENTS AND TRANS-ACTING FACTORS

    • Eukaryotic RNA polymerases and basal transcription factors

    • Diversity in core promoter elements

    • Diversity in general transcription factors

    • Proximal & Distal Promoter Elements, Enhancers and Silencers, Gene-specific Regulators

    4

    2

    DOMAIN STRUCTURE OF EUKARYOTIC TRANSCRIPTION FACTORS

    • Transcription factors – DNA binding domains

    • Transcription factors – transcription activation domain

    2

    3

    ROLE OF CHROMATIN IN EUKARYOTIC GENE REGULATION

    • Role of chromatin in eukaryotic gene regulation

    • Role of histones in eukaryotic gene regulation

    • Role of DNA methylation in eukaryotic gene regulation

    • Chromatin remodelling & gene regulation

    4

    4

    SYNTHESIS OF mRNA, rRNA & tRNA

    • mRNA processing – Role of RNA Pol II in mRNA capping  and mRNA splicing

    • mRNA processing – Role of RNA Pol II in polyadenylation & mRNA editing

    • Regulation of RNA Pol I transcription

    • Regulation of RNA Pol III transcription

    4

    5

    REGULATION OF GENE EXPRESSION VIA CELL SURFACE RECEPTORS

    • Signal Transduction Pathways - Introduction

    • Regulation of gene expression by cyclicAMP

    • Regulation of gene expression by second messengers other than cAMP

    • Regulation of gene expression by Protein Kinase C

    • Regulation of gene expression by Growth factors

    • Regulation of gene expression by cytokines

    6

    6

    REGULATION OF GENE EXPRESSION BY INTRACELLULAR RECEPTORS

    • Regulation of gene expression by steroid hormones

    • Regulation of gene expression by type II nuclear receptors

    • Mechanism of transcriptional activation by nuclear receptors

    3

    7

    REGULATION OF GENE EXPRESSION DURING DEVELOPMENT

    • Gene Regulation during Drosophila Development

    • Signal transduction pathways involved in embryonic development

    • Homeotic genes

    • Epigenetic regulation of gene expression during development

    • Embryonic stem cells and Transcription factor-mediated epigenetic reprogramming

    5

    8

    RECOMBINANT PROTEIN EXPRESSION SYSTEMS

    • Cloning and Expression vectors

    • Eukaryotic protein expression systems - I

    • Eukaryotic protein expression systems - II

    • Eukaryotic protein expression systems – III: Gene expression in mammalian cells using viral vectors

    4

    9

    GENE THERAPY AND TRANSGENIC TECHNOLOGY

    • Human Gene Therapy

    • DNA vaccines

    • Transgenic animals

    • Transgenic plants

    • Knockout mice

    5

    10

    RECENT ADVANCES IN EUKARYOTIC GENE EXPRESSION

    • Regulation of Eukaryotic Gene Expression by Small RNAs (RNA Interference, RNAi)

    • Genomics & Proteomics

    • Metabolic Engineering & Synthetic Biology

  • Lectures
    Eukaryotic Gene Expression - basics and benefits - Lecture 1 - Prof. P.N. RangarajanView
    Eukaryotic Gene Expression - basics and benefits - Lecture 2 - Prof. P.N. RangarajanView
    Eukaryotic Gene Expression - basics and benefits - Lecture 3 - Prof. P.N. RangarajanView
    Eukaryotic Gene Expression - basics and benefits - Lecture 4 - Prof. P.N. RangarajanView
    Eukaryotic Gene Expression - basics and benefits - Lecture 5 - Prof. P.N. RangarajanView
    Eukaryotic Gene Expression - basics and benefits - Lecture 6 - Prof. P.N. RangarajanView
    Eukaryotic Gene Expression - basics and benefits - Lecture 7 - Prof. P.N. RangarajanView
    Eukaryotic Gene Expression - basics and benefits - Lecture 8 - Prof. P.N. RangarajanView
    Eukaryotic Gene Expression - basics and benefits - Lecture 9 - Prof. P.N. RangarajanView
    Eukaryotic Gene Expression - basics and benefits - Lecture 10 - Prof. P.N. RangarajanView
    Eukaryotic Gene Expression - basics and benefits - Lecture 11 - Prof. P.N. RangarajanView
    Eukaryotic Gene Expression - basics and benefits - Lecture 12 - Prof. P.N. RangarajanView
    Eukaryotic Gene Expression - basics and benefits - Lecture 13 - Prof. P.N. RangarajanView
    Eukaryotic Gene Expression - basics and benefits - Lecture 14 - Prof. P.N. RangarajanView
    Eukaryotic Gene Expression - basics and benefits - Lecture 15 - Prof. P.N. RangarajanView
    Eukaryotic Gene Expression - basics and benefits - Lecture 16 - Prof. P.N. RangarajanView
    Eukaryotic Gene Expression - basics and benefits - Lecture 17 - Prof. P.N. RangarajanView
    Eukaryotic Gene Expression - basics and benefits - Lecture 18 - Prof. P.N. RangarajanView
    Eukaryotic Gene Expression - basics and benefits - Lecture 19 - Prof. P.N. RangarajanView
    Eukaryotic Gene Expression - basics and benefits - Lecture 20 - Prof. P.N. RangarajanView
    Eukaryotic Gene Expression - basics and benefits - Lecture 21 - Prof. P.N. RangarajanView
    Eukaryotic Gene Expression - basics and benefits - Lecture 22 - Prof. P.N. RangarajanView
    Eukaryotic Gene Expression - basics and benefits - Lecture 23 - Prof. P.N. RangarajanView
    Eukaryotic Gene Expression - basics and benefits - Lecture 24 - Prof. P.N. RangarajanView
    Eukaryotic Gene Expression - basics and benefits - Lecture 25 - Prof. P.N. RangarajanView
    Eukaryotic Gene Expression - basics and benefits - Lecture 26 - Prof. P.N. RangarajanView
    Eukaryotic Gene Expression - basics and benefits - Lecture 27 - Prof. P.N. RangarajanView
    Eukaryotic Gene Expression - basics and benefits - Lecture 28 - Prof. P.N. RangarajanView
    Eukaryotic Gene Expression - basics and benefits - Lecture 29 - Prof. P.N. RangarajanView
    Eukaryotic Gene Expression - basics and benefits - Lecture 30 - Prof. P.N. RangarajanView
    Eukaryotic Gene Expression - basics and benefits - Lecture 31 - Prof. P.N. RangarajanView
    Eukaryotic Gene Expression - basics and benefits - Lecture 32 - Prof. P.N. RangarajanView
    Eukaryotic Gene Expression - basics and benefits - Lecture 33 - Prof. P.N. RangarajanView
    Eukaryotic Gene Expression - basics and benefits - Lecture 34 - Prof. P.N. RangarajanView
    Eukaryotic Gene Expression - basics and benefits - Lecture 35 - Prof. P.N. RangarajanView
    Eukaryotic Gene Expression - basics and benefits - Lecture 36 - Prof. P.N. RangarajanView
    Eukaryotic Gene Expression - basics and benefits - Lecture 37 - Prof. P.N. RangarajanView
    Eukaryotic Gene Expression - basics and benefits - Lecture 38 - Prof. P.N. RangarajanView
    Eukaryotic Gene Expression - basics and benefits - Lecture 39 - Prof. P.N. RangarajanView
    Eukaryotic Gene Expression - basics and benefits - Lecture 40 - Prof. P.N. RangarajanView
  • DescriptionTypeLink
    CIS-ACTING ELEMENTS AND TRANS-ACTING FACTORSDownloadClick
    DOMAIN STRUCTURE OF EUKARYOTIC TRANSCRIPTION FACTORSDownloadClick
    ROLE OF CHROMATIN IN EUKARYOTIC GENE REGULATIONDownloadClick
    SYNTHESIS OF mRNA, rRNA & tRNADownloadClick
    REGULATION OF GENE EXPRESSION VIA CELL SURFACE RECEPTORSDownloadClick
    REGULATION OF GENE EXPRESSION BY INTRACELLULAR RECEPTORSDownloadClick
    REGULATION OF GENE EXPRESSION DURING DEVELOPMENTDownloadClick
    RECOMBINANT PROTEIN EXPRESSION SYSTEMSDownloadClick
    GENE THERAPY AND TRANSGENIC TECHNOLOGYDownloadClick
    RECENT ADVANCES IN EUKARYOTIC GENE EXPRESSIONDownloadClick
  • DescriptionTypeLink
    CIS-ACTING ELEMENTS AND TRANS-ACTING FACTORSDownloadClick
    CIS-ACTING ELEMENTS AND TRANS-ACTING FACTORSDownloadClick
    CIS-ACTING ELEMENTS AND TRANS-ACTING FACTORSDownloadClick
    CIS-ACTING ELEMENTS AND TRANS-ACTING FACTORSDownloadClick
    DOMAIN STRUCTURE OF EUKARYOTIC TRANSCRIPTION FACTORSDownloadClick
    DOMAIN STRUCTURE OF EUKARYOTIC TRANSCRIPTION FACTORSDownloadClick
    ROLE OF CHROMATIN IN EUKARYOTIC GENE REGULATIONDownloadClick
    ROLE OF CHROMATIN IN EUKARYOTIC GENE REGULATIONDownloadClick
    ROLE OF CHROMATIN IN EUKARYOTIC GENE REGULATIONDownloadClick
    ROLE OF CHROMATIN IN EUKARYOTIC GENE REGULATIONDownloadClick
    SYNTHESIS OF mRNA, rRNA & tRNADownloadClick
    SYNTHESIS OF mRNA, rRNA & tRNADownloadClick
    SYNTHESIS OF mRNA, rRNA & tRNADownloadClick
    REGULATION OF GENE EXPRESSION VIA CELL SURFACE RECEPTORSDownloadClick
    REGULATION OF GENE EXPRESSION VIA CELL SURFACE RECEPTORSDownloadClick
    REGULATION OF GENE EXPRESSION VIA CELL SURFACE RECEPTORSDownloadClick
    REGULATION OF GENE EXPRESSION VIA CELL SURFACE RECEPTORSDownloadClick
    REGULATION OF GENE EXPRESSION VIA CELL SURFACE RECEPTORSDownloadClick
    REGULATION OF GENE EXPRESSION VIA CELL SURFACE RECEPTORSDownloadClick
    REGULATION OF GENE EXPRESSION BY INTRACELLULAR RECEPTORSDownloadClick
    REGULATION OF GENE EXPRESSION BY INTRACELLULAR RECEPTORSDownloadClick
    REGULATION OF GENE EXPRESSION BY INTRACELLULAR RECEPTORSDownloadClick
    REGULATION OF GENE EXPRESSION DURING DEVELOPMENTDownloadClick
    REGULATION OF GENE EXPRESSION DURING DEVELOPMENTDownloadClick
    REGULATION OF GENE EXPRESSION DURING DEVELOPMENTDownloadClick
    REGULATION OF GENE EXPRESSION DURING DEVELOPMENTDownloadClick
    RECOMBINANT PROTEIN EXPRESSION SYSTEMSDownloadClick
    RECOMBINANT PROTEIN EXPRESSION SYSTEMSDownloadClick
    RECOMBINANT PROTEIN EXPRESSION SYSTEMSDownloadClick
    RECOMBINANT PROTEIN EXPRESSION SYSTEMSDownloadClick
    GENE THERAPY AND TRANSGENIC TECHNOLOGYDownloadClick
    GENE THERAPY AND TRANSGENIC TECHNOLOGYDownloadClick
    GENE THERAPY AND TRANSGENIC TECHNOLOGYDownloadClick
    GENE THERAPY AND TRANSGENIC TECHNOLOGYDownloadClick
    GENE THERAPY AND TRANSGENIC TECHNOLOGYDownloadClick
    RECENT ADVANCES IN EUKARYOTIC GENE EXPRESSIONDownloadClick
    RECENT ADVANCES IN EUKARYOTIC GENE EXPRESSIONDownloadClick
    RECENT ADVANCES IN EUKARYOTIC GENE EXPRESSIONDownloadClick
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