Outperforming existing technologies, 'Capture Sequencing' (CaptureSeq) can accurately measure the activity of many specific genes in a sample -- even when they are expressed at minute levels. This will have immediate practical applications, including the diagnosis of many blood cancers.
Publishing in the journal Nature Methods, an Australian team demonstrated the strengths of CaptureSeq.
Dr Tim Mercer, Dr Michael Clark, Professor John Mattick and Associate Professor Marcel Dinger from Sydney's Garvan Institute of Medical Research, who developed CaptureSeq, compared it with techniques currently in use for gene expression analysis, and found it to be much more sensitive to genes expressed at very low levels.
Until recently, it was thought that humans have around 20,000 genes -- that is, the sections of DNA that are 'transcribed' into RNA molecules, then 'translated' into the proteins that perform tasks in cells.
Non-protein-coding genes perform an important regulatory function in various aspects of human development and brain function. Most are expressed only in a few cells rather than whole tissues, or they are expressed at very low levels, making them difficult to study.
The new study shows that CaptureSeq will help us uncover these rare genes by allowing us to explore specific stretches of the human genome at much higher resolution than current RNA sequencing approaches.
At present, patients suspected of having leukemia are tested using existing amplification-based technology, which can search for only one fusion gene at a time. Patients would rarely, if ever, be tested for all 200.
CaptureSeq can also be used to analyse a range of solid cancers and other diseases.
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